Internal Structure Observation based on Serial Sectioning Technique using Riken Micro Slicer System
||Research Subjects and Results
A three-dimensional (3D) internal structure observation system based on serial sectioning was developed from precision cutting devices and optical microscopes combined with a high-precision positioning machine. The precision cutting technique can create mirrored surfaces suitable for the optical microscopic observation. Serial sectioning observations progressed automatically by means of the numerical control in the system. In this study, this system was used to observe defects in industrial materials and microstructure of the biological tissue such as bone on the scale of several tens of micrometers. And microscopic elemental analysis by X-ray fluorescence analyzer was conducted in the system. This system enables automated investigation of the 3D internal structure of materials as well as the identification of their elemental components.
K. Fujisaki, H. Yokota, N. Furushiro, S. Komatani, S. Ohzawa, Y. Sato, D. Matsunaga, R. Himeno, T. Higuchi, and A. Makinouchi
「Three-dimensional microscopic elemental analysis using an automated high-precision serial sectioning system」
Microscopy and Microanalysis, Vol.17(2), 2011(in press)
K. Fujisaki, H. Yokota, N. Hiroki, Y. Yamagata, T. Nishikawa, T. Udagawa, and A. Makinouchi
「Observation of three-dimensional internal structure of steel materials by means of serial sectioning with ultrasonic elliptical vibration cutting」
Journal of Microscopy, Vol.237(1), pp. 89-95, 2010.
K. Fujisaki, H. Yokota, N. Furushiro, Y. Yamagata, T. Taniguchi, R. Himeno, A. Makinouchi, and T. Higuchi
「Development of ultra-fine-grain binderless cBN tool for precision cutting of ferrous materials」
Journal of Materials Processing Technology, Vol.209(15-16), pp. 5646-5652, 2009.
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