Structural changes of actin cytoskeleton controlled focal adhesion on micropatterned substrate
Change in crawling direction of fish keratocytes induced by substrate deformation
Observation and dynamic imaging of actin filament polymerization
Evaluation of changes in mechanical properties of trabecular bone by bone remodeling simulation
||Research Subjects and Results
We have conducted experimental tests for osteoblastic cells and migrating cells focusing on changes in structure, formation process and polymerization speed of actin cytoskeleton induced by change in pericellular biomechanical condition. For example, to observe change in the crawling direction of fish keratocyte due to perturbation of the mechanical condition of the actin cytoskeleton, distribution of focal adhesions was controlled using a micropatterned substrate and compressive strain was applied to the cells. Results of the experiments indicated that the crawling direction depends on the distribution of focal adhesions and the applied strain enhances polymerization and depolymerization rates of actin filaments. Therefore, it can be concluded that the biomechanical factors' regulation of driving forces and their balance determines the cell shape polarity and directional motility, providing fundamental information for computational cell biomechanics.
We challenge further elucidation of effects of change in tensile force in actin filament on changes in structure and formation process of actincytoskeleton. We also provide additional quantitative evaluation of relationship between polymerization speed of actin filament and displacement of migrating cell, trying feedback of experimental results to computational cell biomechanics.
In addition, we try to expand target biomechanical scales including cell scale and evaluate relationship between activities of bone-related cells and mechanical remodeling phenomenon of bone using by "V-BoneRemodeling" which was released by Computational Biomechanics Team.
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D. Tawara, J. Sakamoto, H. Murakami, N. Kawahara, J. Oda and K. Tomita
「Mechanical therapeutic effects in osteoporotic L1-vertebrae evaluated by nonlinear patient- specific finite element analysis」
Journal of Biomechanical Science and Engineering, Vol. 5, No. 5, (2010), pp. 499-514.
D. Tawara, J. Sakamoto, H. Murakami, N. Kawahara, J. Oda, K. Tomita
「Mechanical evaluation by patient-specific finite element analyses demonstrates therapeutic effects for osteoporotic vertebrae」
Journal of the Mechanical Behavior of Biomedical Materials, Vol. 3, No. 1, (2010), pp. 31-40.
T. Hato, N. Kawahara, K. Tomita, H. Murakami, T. Akamaru, D. Tawara, J. Sakamoto, J. Oda, S. Tanaka
「Finite-element analysis on closing-opening correction osteotomy for angular kyphosis of osteoporotic vertebral fractures」
Journal of Orthopaedic Science, Vol. 12, No. 4, (2007), pp. 354-360.
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