Researchers, Developers and Staffs
Hiroyoshi  AOKI Hiroyoshi AOKI


Research Themes

Microfluidic chip for immunoassay using electrospray deposition method.
Application of finite element method (FEM) simulation on microchannel design.

Research Subjects and Results

I developed microfluidic chips for immunoassay using electrospray deposition (ESD) method. The microfluidic chips were applied for immunoassays of human hepatitis virus B (HBV) and influenza A virus. The microfluidic chip showed a higher sensitivity than conventional methods, ELISA and immunochromatography, from a reduced sample.
To improve intra- and inter- reproducibility of the ESD method, I developed a new quantitative assay method for microspot using discretely counting of fluorescent microbeads. Then capillary moving pattern and speed, and antistatic condition of the mask on the ESD were optimized. Furthermore the chemiluminescent image was corrected with a flat-field. The coefficient of variations (CV) of intra- and inter- reproducibility on deposits were reduced to be under 10%.
I evaluated the microchannel design with a fluid simulation using finite element method.

Future Target

I will study a new microfluidic chip for bioassay using the ESD method and the FEM simulation.


Selected Publications

【Papers】
Hiroyoshi Aoki, Hideaki Takaku, Yutaka Yamagata
Nanomaterials, Micropatterning, and Microfluidic Chip Techniques using Electrospray Deposition Method
RIKEN Joint Retreat 2011, Shizuoka, Japan, Jan.-Feb. (2011)

Aoki H, Yamagata Y.,
Development of Microfluidic Chip for Diagnosis of Influenza A to Improve Discomfort in Nasopharyngeal Specimen Sampling.
American Association for Clinical Chemistry (AACC) 2010, Anaheim, CA, July (2010).

Aoki H, Kodama N, Yutaka Y.
Development of Multiplex Microfluidic Chip for Immunodetection of Influenza A.
International Symposium on Microchemistry and Microsystems (ISMM) 2009, Kanazawa, Japan, Nov. (2009)

Selected Publications Page